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KMID : 0381120150370040315
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Genes and Genomics
2015 Volume.37 No. 4 p.315 ~ p.325
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MicroRNA expression profiles in conventional and micropropagated Dendrobium officinale
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Yang Zhili
Yang Dongfeng
Ding Xianfeng
Gao Yong
Li Donghai
Xu Tao
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Abstract
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MicroRNA (miRNA) is defined as an endogenous ~22 nt noncoding RNA that has critical gene-regulatory functions in plants. Dendrobium officinale is among the most important herbs in traditional Chinese medicine. However, no miRNA of D. officinale has been reported to date. To identify the miRNA in D. officinale and to detect the differentially expressed miRNA between conventional and micropropagated D. officinale plants, miRNA microarray was performed following quantitative real-time RT-PCR validation. A total of 120 miRNAs from 37 miRNA families were identified in both conventional and micropropagated D. officinale plants. Among the total number of miRNAs, 45 miRNAs from six families were differentially expressed, including five up-regulated families (miR156, miR164, miR171, miR827 and miR529) and one down-regulated family (miR167) in micropropagated group. Web microrna designer and gene ontology term-enrichment analyses were applied for prediction and functional analysis of the target genes of differentially expressed miRNA. Such miRNAs are mainly related to hormone stimulus response, biological and metabolic process regulation, formation of intracellular organelle, and nucleic acid binding.
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KEYWORD
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miRNA, Microarray, Dendrobium officinale, Target genes, Gene ontology analysis
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